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Image Search Results
Journal: Molecular horticulture
Article Title: Agrobacterium rhizogenes-mediated marker-free transformation and gene editing system revealed that AeCBL3 mediates the formation of calcium oxalate crystal in kiwifruit.
doi: 10.1186/s43897-023-00077-w
Figure Lengend Snippet: Fig. 2 Characterization of transgenic roots of GUS and eGFP. A-C The GUS staining of nine 5 weeks transgenic hairy roots (A) and enlarged field of vision under optical microscope (B) and stereomicroscope (C) from Actinidia chinensis ‘Hongyang’. B. Bar = 2 mm. C. Bars = 200 μm. D Verification of T-DNA insertion fragment of GUS by PCR amplification. M, marker. WT, wild type. PC, positive control. E The eGFP fluorescence signal in 5 weeks transgenic hairy roots of Actinidia eriantha ‘White’. c, root tip. d, the wild type. Bars = 200 μm. F Western blotting with anti-GFP antibodies of 4 transgenic lines and wild type. Molecular mass markers are shown on the left. The expected size of eGFP is 26.9 KDa. G The expression ratio of eGFP and GUS gene in hairy roots. Data shown are averages ±SD; n > 15
Article Snippet: Chlorophyll was removed by incubating stained leaf tissue in 70% ethanol for 6 h, then 80% ethanol for 6 h, and 100% ethanol for 12 h. The enlarged field of view was observed using an optical microscope (Nikon Eclipse E200) and a
Techniques: Transgenic Assay, Staining, Microscopy, Amplification, Marker, Positive Control, Fluorescence, Western Blot, Expressing